Biochem/physiol Actions

Cell permeable: no

Reversible: no

Product does not compete with ATP.

Primary TargetSubstrate containing the α-secretase cleavage site of β-Amyloid Precursor Protein (APP)

General description

An internally quenched fluorogenic peptide substrate that contains the A-secretase cleavage site of β-amyloid precursor protein (APP). Shown to be an effective substrate for yapsin-2, a glycosyl-phosphatidylinositol-linked aspartyl protease found in yeast (V_max/K_m = 0.031 min^-1). The cleavage has been shown to occur at the Lys-Leu bond. Excitation max: ~340 nm; emission max: ~490 nm.

An internally quenched fluorogenic peptide substrate containing the A-secretase cleavage site of β-Amyloid Precursor Protein (APP). Shown to be a substrate for yapsin 2, a glycosyl-phosphatidylinositol-linked yeast aspartyl protease (V_max/K_m = 0.031 min^-1). The cleavage is shown to occur at the Lys-Leu bond.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Komano, H., et al. 1999. J. Biol. Chem. 274, 24431.Komano, H., et al. 1998. J. Biol. Chem. 273, 31648.

Packaging

1 mg in Plastic ampoule

Packaged under inert gas

Sequence

Ac-Arg-Glu(EDANS)-Val-His-His-Gln-Lys-Leu-Val-Phe-Lys(DABCYL)-Arg-OH

Warning

Toxicity: Standard Handling (A)